Glycine Betaine Monooxygenase, an Unusual Rieske-Type Oxygenase System, Catalyzes the Oxidative N -Demethylation of Glycine Betaine in Chromohalobacter salexigens DSM 3043
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چکیده
منابع مشابه
Glycine betaine transmethylase mutant of Pseudomonas aeruginosa.
The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microo...
متن کاملGenetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
Synthesis of the compatible solute glycine betaine confers a considerable degree of osmotic stress tolerance to Bacillus subtilis. This osmoprotectant is produced through the uptake of the precursor choline via the osmotically inducible OpuB and OpuC ABC transporters and a subsequent two-step oxidation process by the GbsB and GbsA enzymes. We characterized a regulatory protein, GbsR, controllin...
متن کاملThermoprotection by glycine betaine and choline.
Glycine betaine is mostly known as an osmoprotectant. It is involved in the osmotic adaptation of eukaryotic and bacterial cells, and accumulates up to 1 M inside cells subjected to an osmotic upshock. Since, like other osmolytes, it can act as a protein stabilizer, its thermoprotectant properties were investigated. In vitro, like protein chaperones such as DnaK, glycine betaine and choline pro...
متن کاملGlycine betaine transport in the obligate halophilic archaeon Methanohalophilus portucalensis.
Transport of the osmoprotectant glycine betaine was investigated using the glycine betaine-synthesizing microbe Methanohalophilus portucalensis (strain FDF1), since solute uptake for this class of obligate halophilic methanogenic Archaea has not been examined. Betaine uptake followed a Michaelis-Menten relationship, with an observed K(t) of 23 microM and a V(max) of 8 nmol per min per mg of pro...
متن کاملThe Oxidative Demethylation of Sarcosine to Glycine
The coupled feeding of sarcosine (N-methylglycine) and benzoic acid, like the coupled feeding of glycine and benzoic acid, results in the excretion of hippuric acid at a rate greater than that when benzoic acid is fed alone (1). When sarcosine containing an excess of N16 is fed to rats, the isotope content of the amino acids of the tissues is almost identical with that of animals fed glycine co...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2018
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00377-18